Trends in Cardiovascular Medicine
Volume 17, Issue 2 , Pages 43-48 , February 2007

Proteomics and Metabolomics Combined in Cardiovascular Research

  • Manuel Mayr

      Affiliations

    • Corresponding Author InformationAddress correspondence to: Dr. Manuel Mayr, Cardiovascular Division, King's College, University of London, Bessemer Road, London SE5 9PJ, UK. Tel.: (+44) 20 3299 3036; fax: (+44) 20 3299 3510.
  • ,
  • Basetti Madhu
  • ,
  • Qingbo Xu

References 

  1. Anderson NL, Anderson NG. The human plasma proteome: History, character, and diagnostic prospects. Mol Cell Proteomics. 2002;1:845–867
  2. Arrell DK, Neverova I, Van Eyk JE. Cardiovascular proteomics: Evolution and potential. Circ Res. 2001;88:763–773
  3. Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997;275:964–967
  4. Bernal-Mizrachi C, Gates AC, Weng S, et al. Vascular respiratory uncoupling increases blood pressure and atherosclerosis. Nature. 2005;435:502–506
  5. Blagoev B, Kratchmarova I, Ong SE, et al. A proteomics strategy to elucidate functional protein–protein interactions applied to EGF signaling. Nat Biotechnol. 2003;21:315–318
  6. Caruso M, Maitan MA, Bifulco G, et al. Activation and mitochondrial translocation of protein kinase C delta are necessary for insulin stimulation of pyruvate dehydrogenase complex activity in muscle and liver cells. J Biol Chem. 2001;276:45088–45097
  7. Churchill EN, Murriel CL, Chen CH, et al. Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation. Circ Res. 2005;97:78–85
  8. Edmondson RD, Vondriska TM, Biederman KJ, et al. Protein kinase C epsilon signaling complexes include metabolism- and transcription/translation-related proteins: Complimentary separation techniques with LC/MS/MS. Mol Cell Proteomics. 2002;1:421–433
  9. Gorg A, Weiss W, Dunn MJ. Current two-dimensional electrophoresis technology for proteomics. Proteomics. 2004;4:3665–3685
  10. Gray MO, Zhou HZ, Schafhalter-Zoppoth I, et al. Preservation of base-line hemodynamic function and loss of inducible cardioprotection in adult mice lacking protein kinase C epsilon. J Biol Chem. 2004;279:3596–3604
  11. Gygi SP, Rochon Y, Franza BR, Aebersold R. Correlation between protein and mRNA abundance in yeast. Mol Cell Biol. 1999;19:1720–1730
  12. Hansson GK, Libby P, Schonbeck U, Yan ZQ. Innate and adaptive immunity in the pathogenesis of atherosclerosis. Circ Res. 2002;91:281–291
  13. Hillebrands JL, Klatter FA, Van Den Hurk BM, et al. Origin of neointimal endothelium and alpha-actin-positive smooth muscle cells in transplant arteriosclerosis. J Clin Invest. 2001;107:1411–1422
  14. Horn M. Cardiac magnetic resonance spectroscopy: A window for studying physiology. Methods Mol Med. 2006;124:225–248
  15. Hristov M, Weber C. Endothelial progenitor cells: Characterization, pathophysiology, and possible clinical relevance. J Cell Mol Med. 2004;8:498–508
  16. Hu Y, Mayr M, Metzler B, et al. Both donor and recipient origins of smooth muscle cells in vein graft atherosclerotic lesions. Circ Res. 2002;91:e13–e20
  17. Huber LA, Pfaller K, Vietor I. Organelle proteomics: Implications for subcellular fractionation in proteomics. Circ Res. 2003;92:962–968
  18. Inagaki K, Hahn HS, Dorn II GW, Mochly-Rosen D. Additive protection of the ischemic heart ex vivo by combined treatment with delta-protein kinase C inhibitor and epsilon-protein kinase C activator. Circulation. 2003;108:869–875
  19. Kirschenlohr HL, Griffin JL, Clarke SC, et al. Proton NMR analysis of plasma is a weak predictor of coronary artery disease. Nat Med. 2006;12:705–710
  20. Leitges M, Mayr M, Braun U, et al. Exacerbated vein graft arteriosclerosis in protein kinase Cdelta-null mice. J Clin Invest. 2001;108:1505–1512
  21. Loscalzo J. Proteomics in cardiovascular biology and medicine. Circulation. 2003;108:380–383
  22. Macri J, Rapundalo ST. Application of proteomics to the study of cardiovascular biology. Trends Cardiovasc Med. 2001;11:66–75
  23. Mayr M, Siow R, Chung YL, et al. Proteomic and metabolomic analysis of vascular smooth muscle cells: Role of PKCdelta. Circ Res. 2004;94:e87–e96
  24. Mayr M, Chung YL, Mayr U, et al. Loss of PKC-delta alters cardiac metabolism. Am J Physiol Heart Circ Physiol. 2004;287:H937–H945
  25. Mayr M, Metzler B, Chung YL, et al. Ischemic preconditioning exaggerates cardiac damage in PKC-delta null mice. Am J Physiol Heart Circ Physiol. 2004;287:H946–H956
  26. Mayr M, Chung YL, Mayr U, et al. Proteomic and metabolomic analyses of atherosclerotic vessels from apolipoprotein E-deficient mice reveal alterations in inflammation, oxidative stress, and energy metabolism. Arterioscler Thromb Vasc Biol. 2005;25:2135–2142
  27. McGregor E, Dunn MJ. Proteomics of heart disease. Hum Mol Genet. 2003;12(Spec No 2):R135–R144
  28. Pandey A, Mann M. Proteomics to study genes and genomes. Nature. 2000;405:837–846
  29. Ping P, Song C, Zhang J, et al. Formation of protein kinase C epsilon-Lck signaling modules confers cardioprotection. J Clin Invest. 2002;109:499–507
  30. Schneider JE, Tyler DJ, ten Hove M, et al. In vivo cardiac 1H-MRS in the mouse. Magn Reson Med. 2004;52:1029–1035
  31. Shamir R, Shehadeh N, Rosenblat M, et al. Oral insulin supplementation attenuates atherosclerosis progression in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 2003;23:104–110
  32. Simper D, Stalboerger PG, Panetta CJ, et al. Smooth muscle progenitor cells in human blood. Circulation. 2002;106:1199–1204
  33. Tanaka K, Sata M, Hirata Y, Nagai R. Diverse contribution of bone marrow cells to neointimal hyperplasia after mechanical vascular injuries. Circ Res. 2003;93:783–790
  34. Unlu M, Morgan ME, Minden JS. Difference gel electrophoresis: A single gel method for detecting changes in protein extracts. Electrophoresis. 1997;18:2071–2077
  35. Vanden Hoek TL, Becker LB, Shao Z, et al. Reactive oxygen species released from mitochondria during brief hypoxia induce preconditioning in cardiomyocytes. J Biol Chem. 1998;273:18092–18098
  36. Wang Y, Takashi E, Xu M, et al. Downregulation of protein kinase C inhibits activation of mitochondrial K(ATP) channels by diazoxide. Circulation. 2001;104:85–90
  37. Washburn MP, Wolters D, Yates JR. Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol. 2001;19:242–247
  38. Xu Q. The impact of progenitor cells in atherosclerosis. Nat Clin Pract Cardiovasc Med. 2006;3:94–101
  39. Yin X, Mayr M, Xiao Q, et al. Proteomic analysis reveals higher demand for antioxidant protection in embyronic stem cell-derived smooth muscle cells. Proteomics. 2006;6:6437–6446
  40. Zampetaki A, Zhang Z, Hu Y, Xu Q. Biomechanical stress induces IL-6 expression in smooth muscle cells via ras/rac1-p38 MAPK-NFkB signaling pathways. Am J Physiol Heart Circ Physiol. 2005;288:H2946–H2954

PII: S1050-1738(06)00176-9

doi: 10.1016/j.tcm.2006.11.004

Trends in Cardiovascular Medicine
Volume 17, Issue 2 , Pages 43-48 , February 2007