Trends in Cardiovascular Medicine
Volume 17, Issue 3 , Pages 101-105 , April 2007

Endothelial Glycocalyx: Sweet Shield of Blood Vessels

  • Jurgen W. VanTeeffelen

      Affiliations

    • Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
    • Corresponding Author InformationAddress correspondence to: Dr. Jurgen VanTeeffelen, Department of Physiology, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands. Tel.: (+31) 43 3884520; fax: (+31) 43 3884166.
  • ,
  • Judith Brands

      Affiliations

    • Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
  • ,
  • Erik S. Stroes

      Affiliations

    • Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands 1105 AZ
  • ,
  • Hans Vink

      Affiliations

    • Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
    • Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands 1105 AZ

References 

  1. Adamson RH. Permeability of frog mesenteric capillaries after partial pronase digestion of the endothelial glycocalyx. J Physiol. 1990;428:1–13
  2. Adamson RH, Lenz JF, Zhang X, et al. Oncotic pressures opposing filtration across non-fenestrated rat microvessels. J Physiol. 2004;557:889–907
  3. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature. 2001;414:813–820
  4. Constantinescu AA, Vink H, Spaan JA. Elevated capillary tube hematocrit reflects degradation of endothelial cell glycocalyx by oxidized LDL. Am J Physiol Heart Circ Physiol. 2001;280:H1051–H1057
  5. Constantinescu AA, Vink H, Spaan JA. Endothelial cell glycocalyx modulates immobilization of leukocytes at the endothelial surface. Arterioscler Thromb Vasc Biol. 2003;23:1541–1547
  6. Curry FR. Microvascular solute and water transport. Microcirculation. 2005;12:17–31
  7. Desjardins C, Duling BR. Heparinase treatment suggests a role for the endothelial cell glycocalyx in regulation of capillary hematocrit. Am J Physiol. 1990;258:H647–H654
  8. Florian JA, Kosky JR, Ainslie K, et al. Heparan sulfate proteoglycan is a mechanosensor on endothelial cells. Circ Res. 2003;93:e136–e142
  9. Gouverneur M, Berg B, Nieuwdorp M, et al. Vasculoprotective properties of the endothelial glycocalyx: effects of fluid shear stress. J Int Med. 2006;259:393–400
  10. Henry CB, Duling BR. Permeation of the luminal capillary glycocalyx is determined by hyaluronan. Am J Physiol. 1999;277:H508–H514
  11. Henry CB, Duling BR. TNF-alpha increases entry of macromolecules into luminal endothelial cell glycocalyx. Am J Physiol Heart Circ Physiol. 2000;279:H2815–H2823
  12. Hu X, Adamson RH, Liu B, et al. Starling forces that oppose filtration after tissue oncotic pressure is increased. Am J Physiol Heart Circ Physiol. 2000;279:H1724–H1736
  13. Huxley VH, Williams DA. Role of a glycocalyx on coronary arteriole permeability to proteins: evidence from enzyme treatments. Am J Physiol Heart Circ Physiol. 2000;278:H1177–H1185
  14. Jeansson M, Haraldsson B. Glomerular size and charge selectivity in the mouse after exposure to glucosaminoglycan-degrading enzymes. J Am Soc Nephrol. 2003;14:1756–1765
  15. Kindig CA, Sexton WL, Fedde MR, Poole DC. Skeletal muscle microcirculatory structure and hemodynamics in diabetes. Respir Physiol. 1998;111:163–175
  16. Klitzman B, Duling BR. Microvascular hematocrit and red cell flow in resting and contracting striated muscle. Am J Physiol. 1979;237:H481–H490
  17. Michel CC, Curry FE. Microvascular permeability. Physiol Rev. 1999;79:703–761
  18. Mochizuki S, Vink H, Hiramatsu O, et al. Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release. Am J Physiol Heart Circ Physiol. 2003;285:H722–H726
  19. Mulivor AW, Lipowsky HH. Role of glycocalyx in leukocyte-endothelial cell adhesion. Am J Physiol Heart Circ Physiol. 2002;283:H1282–H1291
  20. Mulivor AW, Lipowsky HH. Inflammation- and ischemia-induced shedding of venular glycocalyx. Am J Physiol Heart Circ Physiol. 2004;286:H1672–H1680
  21. Nieuwdorp M, Meuwese MC, Vink H, et al. The endothelial glycocalyx: a potential barrier between health and vascular disease. Curr Opin Lipidol. 2005;16:507–511
  22. Nieuwdorp M, Mooij HL, Kroon J, et al. Endothelial glycocalyx damage coincides with microalbuminuria in type 1 diabetes. Diabetes. 2006;55:1127–1132
  23. Nieuwdorp M, Van Haeften TW, Gouverneur MC, et al. Loss of endothelial glycocalyx during acute hyperglycemia coincides with endothelial dysfunction and coagulation activation in vivo. Diabetes. 2006;55:480–486
  24. Platts SH, Duling BR. Adenosine A3 receptor activation modulates the capillary endothelial glycocalyx. Circ Res. 2004;94:77–82
  25. Pries AR, Secomb TW, Jacobs H, et al. Microvascular blood flow resistance: role of endothelial surface layer. Am J Physiol. 1997;273:H2272–H2279
  26. Pries AR, Secomb TW, Gaehtgens P. The endothelial surface layer. Pflugers Arch. 2000;440:653–666
  27. Rubio-Gayosso I, Platts SH, Duling BR. Reactive oxygen species mediate modification of glycocalyx during ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2006;290:H2247–H2256
  28. Smith ML, Long DS, Damiano ER, Ley K. Near-wall micro-PIV reveals a hydrodynamically relevant endothelial surface layer in venules in vivo. Biophys J. 2003;85:637–645
  29. Tarbell JM, Pahakis MY. Mechanotransduction and the glycocalyx. J Int Med. 2006;259:339–350
  30. van den Berg BM, Vink H, Spaan JA. The endothelial glycocalyx protects against myocardial edema. Circ Res. 2003;92:592–594
  31. van den Berg BM, Spaan JA, Rolf TM, Vink H. Atherogenic region and diet diminish glycocalyx dimension and increase intima-to-media ratios at murine carotid artery bifurcation. Am J Physiol Heart Circ Physiol. 2006;290:H915–H920
  32. VanTeeffelen JW, Dekker S, Fokkema DS, et al. Hyaluronidase treatment of coronary glycocalyx increases reactive hyperemia but not adenosine hyperemia in dog hearts. Am J Physiol Heart Circ Physiol. 2005;289:H2508–H2513
  33. Vink H, Constantinescu AA, Spaan JA. Oxidized lipoproteins degrade the endothelial surface layer: implications for platelet-endothelial cell adhesion. Circulation. 2000;101:1500–1502
  34. Vink H, Duling BR. Identification of distinct luminal domains for macromolecules, erythrocytes, and leukocytes within mammalian capillaries. Circ Res. 1996;79:581–589
  35. Vink H, Duling BR. Capillary endothelial surface layer selectively reduces plasma solute distribution volume. Am J Physiol Heart Circ Physiol. 2000;278:H285–H289
  36. Zhang X, Adamson RH, Curry FR, Weinbaum S. A 1-D model to explore the effects of tissue loading and tissue concentration gradients in the revised Starling principle. Am J Physiol Heart Circ Physiol. 2006;291:H2950–H2964

 This work was supported by grants from the Netherlands Heart Foundation (2005T073 and 2003B181).

PII: S1050-1738(07)00045-X

doi: 10.1016/j.tcm.2007.02.002

Trends in Cardiovascular Medicine
Volume 17, Issue 3 , Pages 101-105 , April 2007